The recently established Deep Mine Microbial Observatory (DeMMO) is a long‐term monitoring network at which we can explore the ecological role of biofilms in fluid‐filled fractures to depths of 1.5 km.

While samples from marine and terrestrial deep subsurface fluids have revealed abundant and diverse microbial life, limited work has described the corresponding biofilms on rock fracture and pore space surfaces. An inherent difficulty in studying subsurface biofilms is that of sample acquisition. Further study is needed to explore the presence of clinical species of those opportunistic pathogens in private well water systems to elucidate the health risk.ĭeep subsurface biofilms are estimated to host the majority of prokaryotic life on Earth, yet fundamental aspects of their ecology remain unknown. This study provided integrated knowledge on the persistence of pathogenic organisms in private well water. Next-generation sequencing (NGS) data indicated that the abundance of bacterial species was significantly increased in water collected in kitchens compared with samples from wells directly. Total coliform, as well as Legionella and mycobacteria genetic markers' concentrations were significantly reduced by 3-minute flushing. Naegleria fowleri target sequence was detected in 16.8% and Escherichia coli was detected in 43.4% of the water samples. Our results confirmed the ubiquitous nature of Legionella (86.7%) and mycobacteria (68.1%) in private well water in the researched area, with gene concentration ranged from 0.60 to 5.53 and 0.67 to 5.95 Log10 of GC/100 mL, respectively. The purpose of this study was to investigate the occurrence of fecal indicator bacteria (FIB) and opportunistic pathogens in private well water in rural areas surrounding New Orleans, Lousiana. Private well water systems in rural areas that are improperly maintained will result in poor drinking water quality, loss of water supply, and pose human health risk. 2017), but very few have studied its occurrence in private well water, except one study conducted by Bright et al. fowleri in surface water, geothermally heated water (hot spring), and other thermally impacted waters such as power plant cooling reservoirs (Niyyati et al. To the best of our knowledge, many studies have looked at the occurrence of N. , 2018, much less information is available on the bacterial diversity in salt-marsh groundwater samples. 2018), as well as the occurrences of opportunistic pathogens in fresh water systems (Huang et al. Although bacterial community diversity has been previously studied extensively in groundwater used for drinking in various locations with or without contamination (Cho & Kim 2000 Chang et al. The study shows that a mixed groundwater-aquifer sediment (slurry) sample collected from monitoring wells after minimal purging can be used to assess the aquifer ETBE biodegradation potential at ETBE-release sites using this function-based concept. However, the detection of the ethB gene is a more appropriate function-based indicator of ETBE biodegradation potential than taxonomic analysis of the microbial community. Members of the Comamonadaceae and Gammaproteobacteria families were identified as responders for ETBE biodegradation. The copy number of ethB genes varied with borehole purge volume at the field sites. ETBE biodegradation potential (as determined by quantification of the ethB gene) was detected predominantly in the attached microbial communities and was below detection limit in the groundwater communities. In this study, the distribution of ethyl tert -butyl ether (ETBE)-degrading organisms and ETBE biodegradation potential was investigated in laboratory microcosm studies and mixed groundwater-aquifer sediment samples obtained from pumped monitoring wells at ETBE-impacted sites. However, the distribution of GEO-degrading microorganisms between the groundwater and aquifer sediment must be understood to interpret this potential. Groundwater is often sampled at gasoline ether oxygenate (GEO)-impacted sites to assess the potential biodegradation of organic constituents. Microbes in aquifers are present suspended in groundwater or attached to the aquifer sediment.

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